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Faculty of Health Sciences

McMaster Genomics Facility

10X Single-Cell Sequencing

Single Cell Sequencing

Single Cell RNA sequencing at our facility is done through the 10X Genomics platform which offers multiple different assay options for uncovering gene expression in unique or rare cell populations within a heterogeneous sample, under various conditions and cell states. The Chromium X Controller uses Next GEM technology that allows thousands of cells per run to be individually barcoded before downstream library construction. Once sequenced with the Illumina NextSeq2000, reads can then be mapped back to individual cells via their unique barcode.

10X Assays offered include:

  • 3’ gene expression
  • 5’ gene expression
  • Immune profiling (TCR and/or BCR)
  • ATAC sequencing
  • Fixed RNA Profiling (cells or nuclei)

High viability cells are required for most assays.

Costs will depend on which kit is used and the number of samples. Please email to inquire and we will be happy to put together a quote.