Currently, Mobix Lab orders oligos from the Integrated DNA Technologies (IDT) Portal.

Oligo synthesis proceeds in the 3′ to 5′ direction with the 3′ base covalently attached to a solid support and utilizing cyanoethyl phosphoramidite nucleosides. As the coupling efficiency of each base addition step is less than 100% (normally between 98% and 99%) there is always a portion of short terminated oligos after synthesis.

Purification	Size	% full length (at 98% coupling efficiency)	Applications
Desalting	20mer 30mer 40mer 50mer 60mer 70mer 80mer 90mer 100mer	65 53 43 35 30 24 20 16 13	Sequencing, PCR 50 bases, desalt not recommended (order Ultramers instead)
HPLC	10 – 100mer	Approx. 85%	Mutagenesis, cloning, any other applications

Purification

Desalted oligos will contain the full-length oligo, as well as the smaller failed synthesis DNA fragments, which were capped due to failed incorporation of the next base. These oligos are adequate for most applications, such as DNA sequencing, PCR amplification, etc.

HPLC achieves a higher purity level (approximately 85%) but with a significant decrease in yield.

PAGE purified oligos are the most pure (>90%) but yield is also quite low.

ULTRAMERS are more pure than standard desalted oligos, and recommended for NGS adapter orders.

Modifications

For information about all DNA and RNA oligo modifications available from IDT, please visit Integrated DNA Technologies:

Degenerate Primers

Degenerate oligos are synthesized to contain equimolar mixtures of two or more different bases at a given position within the sequence. Mixed bases are often incorporated into oligonucleotide probes designed to hybridize to an unknown gene that encodes a known amino acid sequence. Since the genetic code is degenerate (e.g. Histidine could be encoded by CAC or CAT), the oligo probe is prepared with C or T at the degenerate site. Oligos with mixed bases are also useful in random mutagenesis and combinatorial chemistry. Degenerate oligos are produced for no additional charge (except for degenerate bases, where a hand-mixed variant is desired. Check the IUB codes for mixed bases.

IUB Codes

Code	Definition	Mnemonic
A	Adenine	A
C	Cytosine	C
G	Guanine	G
T	Thymine	T
R	AG	puRine
Y	CT	pYrimidine
K	GT	Keto
M	AC	aMino
S	GC	Strong
W	AT	Weak
B	CGT	Not A
D	AGT	Not C
H	ACT	Not G
V	ACG	Not T
N	AGCT	aNy